Viral vector production room
Interact with each station to learn more
• CTS LV-MAX Transfection Kit • CTS LV-MAX Production Medium • Forma Steri-Cult CO Incubator • EVOS XL Core Imaging System • HyPerforma DynaDrive Single-Use Bioreactor • Countess 3 FL Automated Cell Counter • Herasafe 2030i Biological Safety Cabinet
QC/analytics room
Centralized expansion room
Prep station
Cryopreservation and cryostorage room
Cell processing and engineering area
Sterile preparation area—BSC wall
• TSX Series High-Performance Undercounter Lab Refrigerator • QuantStudio 5 Real-Time PCR System, 96-well, 0.1 mL • Attune NxT Flow Cytometer • CytKick Max Autosampler
• HyPerforma GXCore Bioprocess Controller, for HyPerforma Rocker and Single-Use Bioreactors (GMP) • HyPerforma Rocker Bioreactor • Forma Steri-Cult CO Incubator
• Herasafe 2030i Biological Safety Cabinet • TSX Series High-Performance Pharmacy Refrigerator • Forma Steri-Cult CO Incubator
• CryoMed Controlled-Rate Freezer • TSX Series Ultra-Low Freezer • CryoExtra High-Efficiency Cryogenic Storage System
• Herasafe 2030i Biological Safety Cabinet • CTS OpTmizer T-Cell Expansion SFM • CTS Dynabeads CD3/CD28
• CTS DynaCellect Magnetic Separation System • CTS Rotea Counterflow Centrifugation System • CTS Xenon Electroporation System
T cell manufacturing for cell therapy applications
• PureQuant CD8+ T-Cell Assay • MycoSEQ Mycoplasma Detection Kit • Sorvall X4 R Pro Centrifuge Series • Countess 3 FL Automated Cell Counter
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Cat. No.
A4132601
The Gibco CTS LV-MAX Transfection Kit is an essential component of the CTS LV-MAX lentiviral production system. Based on extensive design of experiment (DOE) methodologies, the cationic lipid–based transfection reagent, lentiviral production supplement, and LV enhancer are specifically screened for maximal delivery of multiple DNA plasmids to achieve the highest possible lentiviral titer when used in conjunction with CTS Viral Production Cells cultured in CTS LV-MAX Production Medium.
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Process development room:
The Gibco CTS LV-MAX Production Medium is a chemically defined, serum-free, protein-free medium validated for recombinant lentiviral vector production. In addition to supporting high cell density suspension cultures of CTS Viral Production Cells (HEK293F- derived cells), this medium is suitable for preclinical-, clinical-, and commercial-scale transient transfection and lentiviral production when used with the CTS LV-MAX Transfection Kit. The CTS LV-MAX Production Medium undergoes stringent quality control testing to suit various manufacturing work streams for cell and gene therapy applications.
CTS LV-MAX Production Medium
A4124001
The Invitrogen Countess 3 FL Automated Cell Counter is a benchtop assay platform equipped with state-of-the-art optics, fully automated focus and lighting capability, and image analysis software driven by a neural network–based machine-learning algorithm, for rapid assessment of primary and immortalized cell lines. The Countess 3 FL counter is capable of brightfield or fluorescence illumination with three-channel flexibility (brightfield and two optional fluorescence channels), enabling researchers to count cells, monitor fluorescent protein expression, gain insights into apoptotic processes, and measure cell viability.
Countess 3 FL Automated Cell Counter
AMQAF2000
The Thermo Scientific HyPerforma DynaDrive Single-Use Bioreactor (S.U.B.) continues the success of the robust HyPerforma bioreactors first launched in 2006 and updated in 2013. The HyPerforma DynaDrive S.U.B. provides improved performance and scalability to larger volumes, and is suitable for fed-batch and perfusion processes. The HyPerforma DynaDrive S.U.B. helps ensure optimal cell culture performance across all scales and increased cell densities.
HyPerforma DynaDrive Single-Use Bioreactor
DDB0050.1011 DDB0050.1021
The Invitrogen EVOS XL Core Imaging System is a digital, transmitted-light, inverted imaging system for cell and tissue culture applications and routine cell maintenance. Its color camera and high-quality optical system deliver high-definition images with exceptional ease. With a small footprint and ergonomic design, the all-in-one EVOS XL system is ideal for basic imaging needs and a perfect addition to any cell culture room or facility.
EVOS XL Core Imaging System
AMEX1000
3310CTS
The Thermo Scientific Herasafe 2030i biological safety cabinet (BSC) leverages a legacy of global leadership in BSC design with breakthrough innovations to optimize contamination prevention, user convenience, and information management. Equipped with smart self-monitoring safety features, an intuitive graphic touchscreen interface, and built-in connectivity to preserve important performance data, the Herasafe 2030i BSC delivers performance with superior precision for demanding cell culture applications and other sensitive work.
Herasafe 2030i Biological Safety Cabinet
51033761
CTS LV-MAX Transfection Kit
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Thermo Scientific Forma Steri-Cult CO Incubators offer the ultimate protection for high-value cultures with every element of their design, from the ISO Class 5 HEPA filtration system to their easy cleaning processes, and proven automated sterilization cycles. As the largest-capacity stackable CO incubator in the market today, the CTS Series supports cell therapy needs from research to high-volume production, providing the documentation critical to validation every step of the way.
Forma Steri-Cult CO Incubator
Products
A29003
The Invitrogen Attune NxT Flow Cytometer has expanded functionalities to drive your research forward. Designed and developed to remove common barriers associated with flow cytometry, the evolutionary capabilities of the Attune NxT Flow Cytometer offer adaptable optical configuration options so you can get the most out of your multicolor analysis.
Room:
The Invitrogen CytKick Max Autosampler is for use with the Attune NxT Acoustic Focusing Cytometer. It enables rapid processing of multiple samples by automatically analyzing samples from 96- and 384-well plates (standard and deep), as well as some custom plate types and microcentrifuge tube racks. The CytKick Max Autosampler features a boost mode that enables faster sample processing, as well as passive cooling for some plates/racks. A CytKick Max Accessory Kit is included that contains four accessory plates: two cooling plates and two tube adaptors, as well as a Max dongle key. A computer is not included with the autosampler since one is provided with the cytometer.
CytKick Max Autosampler
A42973
The Applied Biosystems QuantStudio 5 Real-Time PCR System is designed for users who need superior performance, maximum dye versatility, and security options in a real-time PCR system that is affordable and easy to use. The optimized Design and Analysis software is ideal for both first-time and experienced users. When connected to the Thermo Fisher Connect Platform, the QuantStudio 5 system provides access to your data wherever and whenever you want. Using proven Applied Biosystems OptiFlex technology (featuring 6 decoupled channels and white LED) and featuring six independent Applied Biosystems VeriFlex temperature zones, the QuantStudio 5 96-well system enables improved data accuracy and sensitivity for a broad range of genomic applications. The QuantStudio 5 system also offers the built-in software features of electronic record security and prevention of unauthorized instrument access to assist with 21 CFR Part 11 compliance.
QuantStudio 5 Real-Time PCR System, 96-well, 0.1 mL
A28138
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These Applied Biosystems TaqMan Assays are qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples. Results are provided as a percentage of target cell type out of total input cells, using a free analysis tool.
PureQuant CD8+ T-Cell Assay
A43674
The Thermo Scientific Sorvall X4 R Pro series centrifuges have touchscreens that enable easier programming for quicker results—plus easily access and track all runs, rotor life, and centrifuge health. The Sorvall X4 R Pro series centrifuges have a capacity up to 4 L, including 196 blood tubes and 96 15-mL conical tubes, and auto-lock for fast rotor exchange.
Sorvall X4 R Pro Centrifuge Series
75009920
Designed for your environment, the Thermo Scientific TSX Series high-performance undercounter lab refrigerators support sample protection and sustainability objectives for the storage of media, reagents, and chemotherapy, pharmaceutical, and other research and medical products. Powered by V-drive, the TSX Series delivers low energy usage, decreased noise levels, and improved performance and user experience. These new undercounter refrigerators are quiet and feature an improved user interface, automated locking, and increased storage capacity and reliability.
TSX Series High-Performance Undercounter Lab Refrigerator
TSX4505GA
Attune NxT Flow Cytometer
The Applied Biosystems MycoSEQ Mycoplasma Detection Kit is a real-time PCR assay designed and validated to meet the requirements of European Pharmacopoeia Chapter 2.6.7 and, after validation has been accepted by regulatory agencies for at least 42 on-market products for lot release as of 2021. The primers were designed using a proprietary bioinformatics pipeline to detect >90 mycoplasma species with high specificity and no cross-reactivity to closely related bacterial species. The assay's sensitivity has been demonstrated by internal and external validation to detect 10 CFU/mL or the genomic equivalent of 10 GC/mL, as recommended by regulatory guidance.
MycoSEQ Mycoplasma Detection Kit
4460623
Sorvall X4R Pro Centrifuge Series
Manufacturing room:
HyPerforma Rocker Bioreactor
F100-2683-002
The Thermo Scientific HyPerforma GXCore Bioprocess Controller is an innovative, compact, cost-effective, open-architecture controller built for research and process development applications. The GXCore controller can control most brands of single-use or autoclavable bioreactors or fermenters ≤15 L in size. The controller operates using Thermo Scientific TruBio software, which provides easy data transfer for process scale-up or scale-down and the configurability to modify your control strategy along with your process. Use the device to control parameters such as pH, dissolved oxygen, temperature, stirrer speed, gas mix via mass flow controllers, foam/level, and speed of variable speed pumps with totalizers, as required by most research and process development applications.
HyPerforma GXCore Bioprocess Controller, for HyPerforma Rocker and Single-Use Bioreactor (GMP)
F100-7000-000
The Thermo Scientific HyPerforma Rocker Bioreactor brings control and measurement to rocking bioreactor applications. The rocker is controlled by a Thermo Scientific HyPerforma G3Lab Controller and TruBio software, providing a complete solution for research, process development, or seed train production applications. The rocker is compatible with a 10 L, 20 L, or 50 L Thermo Scientific RockIt BioProcess Container (BPC), providing from 5 L to 25 L of working volume, and integrates a novel TruFluor pH and dissolved oxygen (DO) sensor for combined pH, DO, and temperature monitoring.
TSX Series High-Performance Pharmacy Refrigerator
Thermo Scientific TSX Series high-performance pharmacy refrigerators are designed with features that support sample protection and sustainability objectives for the storage of pharmaceuticals, vaccines, and chemotherapy and other medical and pharmacy-grade storage requiring 2 to 8°C. The Series’ V-drive technology is designed to provide temperature uniformity that continually adapts to user patterns, offering significant energy savings without compromising protection. Now certified to the NSF/ANSI 456 Vaccine Storage standard, the TSX Series delivers the performance and quality you need, in addition to the low sound, low energy usage, and exceptional user experience you deserve.
TSX5005PV
TSCM34PA
For customers in research, cell and gene therapy, vaccine production, and biobanking, the Thermo Scientific CryoMed Controlled-Rate Freezer (CRF) provides precise, repeatable freezing results that protect samples from intracellular freezing. The CryoMed CRF features enhanced data traceability via a user-friendly touchscreen interface and offers customizable freezing profiles, while supporting 21 CFR Part 11 compliance.
The ENERGY STAR certified Thermo Scientific TSX Series ultra-low freezers are designed to meet the highest standards in reliability, sustainability, and temperature management. At the heart of the TSX Series is V-drive technology, designed to adapt to user patterns, substantially reducing energy and HVAC costs without compromising performance. All TSX Series freezers are manufactured in a zero-waste facility and are built with natural refrigerants and water-blown foam insulation, compliant with the U.S. EPA’s Significant New Alternatives Policy (SNAP), the European Union’s F-Gas compliance, and other sustainability standards.
TSX Series Ultra-Low Freezer
TSX60086A
Designed for both liquid- and vapor-phase storage, the Thermo Scientific CryoExtra series features automated temperature monitoring and microprocessor-based LN level control, providing peace of mind for valuable samples.
CryoExtra High-Efficiency Cryogenic Storage System
CE8140
CryoMed Controlled-Rate Freezer
ENERGY STAR is a trademark of the U.S. EPA.
The multipurpose Gibco CTS Rotea Counterflow Centrifugation System offers exceptional flexibility in a cell therapy development and manufacturing system. This versatile system applies the proven counterflow centrifugation method to a broad range of cell processing applications, such as CAR T cell therapy, stem cell therapy, and PBMC isolation. The CTS Rotea system consists of a compact multipurpose instrument, a sterile single-use kit, and intuitive, visual, customizable software. Together they constitute a closed system that offers exceptional cell recovery, flexible input and output volume capability, and high throughput. It’s ideal for multiple processes in your workflow, including cell separation, concentration, washing, buffer exchange, and cryopreservation. Highly concentrated outputs (with volumes as low as 5 mL) contain very little buffer or carryover, making them optimal for autologous cell therapy applications. The compact footprint, process flexibility, and closed-loop flow allow the system to seamlessly scale with your process, from research through commercial manufacturing.
CTS Rotea Counterflow Centrifugation System
A47695
The Gibco CTS DynaCellect Magnetic Separation System is an automated instrument for T cell isolation and activation, as well as magnetic bead removal. The instrument utilizes an integrated magnet-rocker and fluidics management for target-cell separation and subsequent bead removal in a closed system. The key design feature is the user-programmable software, which enables design flexibility, performance precision, and scalability from process development to commercial manufacturing. Together with the fit-for-purpose, sterile, single-use consumables, it delivers high cell purity, recovery, and viability.
CTS DynaCellect Magnetic Separation System
A55867
CTS DynaMag Magnet
The Gibco CTS DynaMag Magnet is suitable for use with commercially available sterile blood and culture bags, tubing, and connectors.
12102
CTS Xenon Electroporation System
The Gibco Cell Therapy Systems (CTS) Xenon Electroporation System is a closed, modular, large-scale platform that offers full control over electroporation parameters for optimal performance. The CTS Xenon Electroporation System delivers superior transfection performance and viability. The system enables electroporation of up to 2.5 x 10 cells in a 25 mL run to support both process development and manufacturing work in clinical cell therapy. The Gibco CTS Xenon SingleShot Electroporation Chamber is designed for rapid electroporation of 1 mL of cells at a recommended concentration range of 20 x 10 to 100 x 10 cells/mL for process development applications, while the Gibco Xenon MultiShot Electroporation Cartridge, designed for clinical research process development, enables consistent electroporation of volumes up to 25 mL in under 25 minutes in a closed, GMP-compliant system. flexible and programmable protocols for a range of cell types and payloads make the CTS Xenon Electroporation System ideal for process development and manufacturing work in clinical cell therapy.
A52727
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Gibco CTS Dynabeads CD3/CD28 are intended for isolation, activation, and expansion of human T cells in translational research. By combining anti-CD3 and anti-CD28 antibodies on Dynabeads magnetic beads, CTS Dynabeads CD3/CD28 provide both the primary and co-stimulatory signals that are required for activation and expansion of T cells.
CTS Dynabeads CD3/CD28
40203D
Gibco CTS OpTmizer T-Cell Expansion SFM supports the growth and expansion of human T lymphocytes. It is a serum-free, xeno-free complete medium consisting of CTS OpTmizer T-Cell Expansion Basal Medium and CTS OpTmizer T-Cell Expansion Supplement, which are mixed together prior to use.
CTS OpTmizer T-Cell Expansion SFM, no phenol red, bottle format
A3705001
The Thermo Scientific Herasafe 2030i biological safety cabinet (BCS) leverages a legacy of global leadership in BSC design with breakthrough innovations to optimize contamination prevention, user convenience, and information management. Equipped with smart self-monitoring safety features, an intuitive graphic touchscreen interface, and built-in connectivity to preserve important performance data, the HeraSafe 2030i BSC delivers performance with superior precision for demanding cell culture applications and other sensitive work.
ex vivo
